TREM2 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	TREM2
Locus ID	54209
Kit Components	KN221132G1, TREM2 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AGTAAGATGAGCAGCCGGAG KN221132G2, TREM2 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AAGGCATCACAGGGCACGGA KN221132-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CAGGGAACAG ACACAGGTAG GTTTTGGATG TGTGGTTACA CACCCAGAAA GTCAGGAAGT CTGGCTCCAG AGCTGTGTAC TTAACTGCTG CCACACTACA GGAATGACAG CCCTGGGGGG ATGAACTAAG AGGTGCTGGA TGAGGGTCCT GGCCTCTAAA GGCACAGCTG TTCTCCAACT CTTGCAAGGC TGAAACCAGA AGATGGCGGG CATTGCAGCT GGTGGAGGGT CTGAATACAG CTGTGAGGAT AGTGATCCCT GGGCTAGGCT CTGCAAGGAA ACTGAGCAGT GCAGGGCCTT ACCAGCCCCA ACCATCTGGG GGCCACCCTG GCTGGCACCA GCAGGAGGGT GGGCTGGCTT CTCAGAGGTC TGGGAGACTC AGCCTCCTTC TGCCAGGGCT GCAGTGGCCG ACTCCTCCTC CCCTCTGTCC CCACCCTGCA CCGCCTCCAG ACCCCAGTCC TGACTATTGC TTAATCCCCA GGAGCCCAGT TCCTGTGGGC AGCGCCTGAC ATGCCTGATC CTCTCTTTTC TGCAGTTCAA GGGAAAGACG AGATCTTGCA CAAGGCACTC TGCTTCTGCC CTTGGCTGGG GAAGGGTGGC AAGGCGGTGG CAGTCCTCGC ATGCCTGAGT GCCCTGTTCT GCACTCCTCT CCTCCCCACC CTGCCCATAT TCTTCACACC CTCCTTCCCT CCCCAGTATC AAAACCTACC CTCAGACTCT TTCTGCATGT GGACATGTAA GGGGCTGGTG GACTCTGGTT GCATTGGAAG GGAAGGAGTG CTAACAGTGG CATCCCAGGC ACTAGCTGGC AGTTGGGGGA AGCTTTGGGG GGCACTGGCA CTGGTAATAG CCTCTGAAAT TATAAGCCAC TAATTATGAG CCCCTACAGT TATAAAGGAG GAAAGAACCT GAGGATGTTC ATCTGCATCT TTGGGGCACT CTCTCCCCTG CTCTGAAGGT CCCCTTGTCC TCAGCTCTTG ATGGGAGGTG AGGAGCGTAG AGATTTCCGT TGCTGAGTTG AGGGGAATTG ACACACCAAA TTTTTGTACA CAATTGCTTC TTCCCGCTGA GGAAAGCGCA CCTTTTGCTC CCCCAGAGCA CTGGACAAAC CTGGGCAAGA GGAGAGACGG TGCCAAATGG AGTCTTGTTC CTGCAGCTTC TTAGATGGTG GTCAGGGGGA AGGCGGGGTT CTGAGGCATG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001271821, NM_018965
Synonyms	TREM-2; Trem2a; Trem2b; Trem2c
Summary	This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.