DKK4 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	DKK4
Locus ID	27121
Kit Components	KN221217G1, DKK4 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TCCTGGACTTCAACAACATC KN221217G2, DKK4 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGAGCTCTGCTGACCTGCAT KN221217-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TCATCCTTTC TTTGAGCACA ATTAGCTGTG AAAACAGATC TTAGGGCAGA CTCCCTCCCT ACCCCCAAAG GATTACTGAA GGTAGGAAAT GCAGGTGATT ATCAGAGTTT GCCTTTGATA CAGACATCCT GCTCTGCCGT GGCCCTTTGA ACTAACTTTG ATATGCAATA ATTAAGAGGG ATTGAACCCC TGGAGGAGAA GCCGCATGGC CCTGCCTCTT CTCTCCTTCT ATTGAGTCCT TGTTTTGAAC TATTGATCAA ACAGATTTGA AGGGATTTGT TGAAGCCTGT GTGGGGCAGG AGGAAGGAAC GGAGCGGGGA GGAGGCACAT CTGGTTATAA ATAGTTTCAG GAGGAACCTG CTGGTCAGAC TTTGCTCAGC CGATTTCACG CACCTTACTC AGACTAAGGT TTACTTCTTT CAGAAAAAGC AGTGACAAAC AGACGACGTG CTGAGCTGCC AGCTTAGTGG AAGCTCTGCT CTGGGTGGAG AGCAGCCTCG CTTTGGTGAC GCACAGTGCT GGGACCCTCC AGGAGCCCCG GGATTGAAGG GGGCCCGGAA GGTAAGGCTG CTAGGGAGGG GGGCGAGGTA CGGGACTGCT GACAGCTGGG GTGTTTTGTT TAGGCTTTAG TAAGTCATTC TGATTCATGT ATGAAATGTC TTACCTAGCA GGTAAATTGA ATTGGTTTCG GATTTGGGAG GGCATCCTGG TAGGATCAGT GGCCTTAGTG TGAATGTATG AAATAAACAC AGTGGCTTTT CACATCATTC GGCCATACGT TTGTCTATAA ATCACGAAAT AAACGAACAC TTTGGAAGCT CTACTATGTG CCAGCTCTTT TACATGTAAT ACGTCATGTA CTCTTCCTCA CAATTTGAGA TAGGTGAAAC ATTCCCTAAT TCCAGATGAG GAAATAAGAC TCAGAGATGT TGAAGAGCTT ACCCAAGCTC ACACAGCCAG CAAGCAAACA TGGGAATGAG AATTTAAACA GAGGGCCATG ATGCATTGCC TTGTCACCAT CTAACTTCAC AGAGAGAGTT GTTTTAGGAA TCGCTTTATG TATGTGTATA TGTTATTATA TATCGTATAG ATTATTACAT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_014420
Synonyms	DKK-4
Summary	This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. [provided by RefSeq, Jul 2008]

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