TBLR1 (TBL1XR1) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
Donor DNA	mBFP-Neo
Symbol	TBLR1
Locus ID	79718
Kit Components	KN222452G1, TBLR1 gRNA vector 1 in pCas-Guide CRISPR vector KN222452G2, TBLR1 gRNA vector 2 in pCas-Guide CRISPR vector KN222452BN-D, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette. Homologous arm and mBFP-Neo sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; mBFP-Neo in green; Right arm in violet ACTGTATTCC AGCCTATCTC ATACATACCT AGAGCTTTTT GAGACTTGAA CATTACAGAT GAGATGGTGA GAAAATAGAG ACTTCATATC CTGCCAAGTT TCATAGATAA AACTGGAGTG GATAACTCAT GGTTCTACCC CACCCAATGG TCTTTTCCCA AGTAACTGAT ATTCTTTTTT AACTAAATCA GCCTTCTTGC AAATAACTAA TATTTTTTAT TTTTAAAATT TACTTCTTTT TACAGTTAAG GAGAAAAAGT GAGCAACATT TTATGCTATT TGATAAAATC ATTAAAATAT TATGCTAAAG CCCTCTAAAA AATTTTGTTT TATTTTTGCC AGTATGTTAC TTTGAAGAAA AATAACACCA GCCATGTTTT GGTTGTATAA TCATTTTCCT AACTATTGGA AGCAACTTTG TCAGGTTTTA TTATTAGGCA ATGTAGAACC TTCGTTTTAC ATCATTTAAT AAAATCAAAT GGTTTTTACA ACATTGTTTA CATCTTGTTT AAAAATATTT TACTGAGAAA ATAATTAACT TTGTTTTATT TTTAGGGTTA TATCCTGTGT TGTGACCTCA TGGTTTAAGT GGGAATAAAG TAAATAGGCC TCAAATTATT TTAATATTCT CAAAATAATC TTTTATGCAT TTGAAATTTG TATCAGTCCA AACCGTTGAA CTTTGTATGT TTTCTTAATG TCTGGACTGC CATTTTAAAG CTATGTTTTA CTGCATGGTT TAACTCTCTG ACTCTTTCAA ATATGTTGCA AACAAGGCAA TTATTTGTAA CAGTTTGGCT TTCGTTGTTC AAAGACCATT TTTATAATCT GTTATGAGGT GAGATTATTG CATATGTAGT AGGAACATAA AAACTTTTAG CCACCATCAG TTTGATAATG TATATCCAGA ATTCTCAATT AAATGTTGTT GGTCCTAATT GTAGTTAGTA TTTTGGAAAT TAAATTAACA GTATTTTCCT AGAATGCTTT CAAAATTATT ATTTGAATTA AGTGTATCCT TGATCTGAAT TGTTATTATT GTAATGGGAG TAATTATAAA TAGAAATCAT TGAAAGCCAT TTGCAACTAA TATTAAATCA ATCAGTGATC TTCCAAGTTT TATTGATAGA TAAAACAGAA GGAATACTGC AAGGGGAAAT GTGGATGGAT TAAGCAAAAA TTTAAAAGTA GAAGAGGGAA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001321193, NM_001321194, NM_001321195, NM_024665
Synonyms	C21; DC42; IRA1; MRD41; TBLR1
Summary	This gene is a member of the WD40 repeat-containing gene family and shares sequence similarity with transducin (beta)-like 1X-linked (TBL1X). The protein encoded by this gene is thought to be a component of both nuclear receptor corepressor (N-CoR) and histone deacetylase 3 (HDAC 3) complexes, and is required for transcriptional activation by a variety of transcription factors. Mutations in these gene have been associated with some autism spectrum disorders, and one finding suggests that haploinsufficiency of this gene may be a cause of intellectual disability with dysmorphism. Mutations in this gene as well as recurrent translocations involving this gene have also been observed in some tumors. [provided by RefSeq, Mar 2016]

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