Dag1 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN304265LP

Dag1 - mouse gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

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CNY 12,260.00


货期*
6周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


Rabbit Polyclonal Anti-Dag1 Antibody
    • 50 ug

CNY 4,628.00


Dag1 (Myc-DDK-tagged) - Mouse dystroglycan 1 (Dag1)
    • 10 ug

CNY 9,808.00

Specifications

Product Data
Format 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
Donor DNA Luciferase-Puro
Symbol Dag1
Locus ID 13138
Kit Components

KN304265G1, Dag1 gRNA vector 1 in pCas-Guide CRISPR vector

KN304265G2, Dag1 gRNA vector 2 in pCas-Guide CRISPR vector

KN304265LP-D, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001276481, NM_001276482, NM_001276485, NM_001276486, NM_001276492, NM_001276493, NM_001276494, NM_010017
Synonyms D9Wsu13e; DG; Dp71; Dp427
Summary This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. A complete lack of the encoded protein in mice results in embryonic lethality due to the disorganization of Reichert's membrane. Chimeric mice deficient in the encoded protein overcome embryonic lethality but develop a progressive muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein. [provided by RefSeq, Nov 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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