Irak3 Mouse Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	Irak3
Locus ID	73914
Kit Components	KN308417G1, Irak3 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AGGTCGAAGAGCAGCAACTG KN308417G2, Irak3 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GCAAAGCTCTCCCAGCAGTG KN308417-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CGACGGCAGG GGTTGCCTTC TGCAGCAAGG ATGAGCAGTT TGTTTGCCCA GGTTTGGCCT GTTTGTTGTG GTTTTCTTTT TATGTCCATT TCGCCCGGCG TGTACACGTT TCCTTCCGTT TAAAGTTATT TGGCAAAAAC GTGTTTCTCG GTGAGCACGC ATTTCTTTCC TGACGGGACA AAGAGAAACT TTAAAAGATG TAACACAAAC CTCTTAGATC CATCGTGGCC AGGAGGCCGC CCGTCCCAGA GCAGCAGAGG GGTGGCGGCT TTCCCGGATT CCAGCCGCTG TTCCCCGCCC TCATTTTCTT GGCTGGTCTC CTGGGTCATG TCCCAAGACG GGGACGGACA GAACGGTTCA TAACAAAGAT GACCTGGGGT GCGGCGCGCA GGGTCGCGGG ACAGCGGCTA GGAGCAAGGG CAGCCCAGGT CTCGCAGGGC GCATCCCTGG GGAGCGGCTC CCTACGTCAC GTGGGGCCCG CCCTGCGACG GCGTGTTGCA AGCTCCGGGC AGCCGTCGTG GAGCTGAATT TCCGCGGTTG TGAAAGGCGG TGACAGCGGC GACCTCCCTG CTTCTCTGCG TGGGGTCCCG GGACTCCGCG TGCGATGGCC CGCTCGGCTG GCGGGGCCTG GGTGAGTGCG ACTCAGACCT GCCGGTCCTC CCCCGCGAGG GACTCAGCAA ACCCGGGGAC CTGCCACTCA CTCTCCTTCC CTCTCTGACA GTGGGGTACT GCTGTATCAA GTTAGGGGAT TGGGTATGTC AGATGTCAAG AAAGCATCCC GTGCTGGGAA GAGATTCTCG CTTCACTCGG TTTGCAGTGT GCGCAGCAGT ATGCAAATAT TACCGTATGC AGGGCATGGC TACTGGCTCT ACAACCCCTA CATCAGATTT TTTTAATATT ATAACTTCTT TTTTGAGATG CCGCTCAGTT GCTAGAGTGC TTACCTATAG ATGTATGAAG CTGCATGTTC GATTCCCTAG CTTGGAATAA AACAGACAGT GGTAGTGCCA GTCATTGAGA GGTAGAAGTA GGCAGGACAG ATGCTCAAGA GCATCCTCTC CTTGTAGAGA GTTCCTAGCT GGCTTGGGAA ACATGAGACG CCATCTCAAA AACAAAAAGC AAACTTACTT TCTTTTCTTT CCTTTCTTTC TTTCTTTCTT TCTTTCTTTC TTTCTTCCTT CCTTCCTTCC TTCCTTCCTT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_028679, NR_152856, NM_001359184
Synonyms	4833428C18Rik; AI563835; IRAK-M
Summary	Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.[UniProtKB/Swiss-Prot Function]

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