Myd88 Mouse Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	Myd88
Locus ID	17874
Kit Components	KN310583G1, Myd88 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GTCTGCGGGAGACCCCCGCG KN310583G2, Myd88 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CCCACGTTAAGCGCGACCAA KN310583-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet CAACGTTAAA GAGTAAGGTG GTAGGTTCCG CGTGTTTTTT GCAGACCCCG GGAAGGTAGT GCTGTTTGTC TCAGAAAACA AAGGACAAGA TACCCTAAAT GCCAATTTAT GGGCAAGGTC GTGGGCTTTC CCCTTTAAAA AGCAGAGTCA CAAGTGACAT CAGATAGCTA AAGGTAGACT GAGCCTTCGG GTTACTACTT CCGAAGCCAG TCTGTCTCTT CCCTCCCTAC TACCAGAATA TAAATTACTG TACAGAAGGG CTGTAACTCC TTCAAGTCCT AAACAAGGCT GATTGAGCTA ACCATGCATA ATAACCACTA CCAGATCGCC TAGTCCATCC ACCTTGATCG GTGGGCCAAG GCAGATGCCA GAGGCGGTTT ATATAAATTC TCACACCTTC GAGGGGAGGT GAGCAAACGT TAGAAGCACA AGTGGGTTGA CTTTTAGGCT GGGTAGTTCT GGCCCCGTAG TCTAGATTCT AGCAAAGGGC GGAGCTTCTC GGAAAGCGAA AGAAGGAGGG GCTGGCAGGA GACTTAAGGG AAGTAGGAAA CTCCACAGGC GAGCGTACTG GACGGCACCG GGGGCCCAGG GTTGCCTGCC ATCGCTGTTC TTGAACCCTC GGACGCCCGT GGCGGCCGAC TGGACCTTGC TGGCGGAGGA GATGGGCTTC GAGTACTTGG AGATCCGAGA GCTGGAAACG CGCCCTGACC CCACTCGCAG TTTGTTGGAT GCCTGGCAGG GGCGCTCTGG CGCGTCTGTC GGCAGGCTGC TAGAGCTGCT GGCCTTGTTA GACCGTGAGG ATATACTGAA GGAGCTGAAG TCGCGCATCG GTGAGGAAAC GTGCTTCCTG GCTTTGTGCC AAGGATCAAC GAGGCTAGCC TTGGCATATT AATCTTGTTC ATTTTTTTCT GGGGTGCGCT AGGGAGGAAG AGAGTGACAG TCGAATTTTC TGTCAGTATG CAGGTCAGCG TAGGGATTGG GAGAGGGGTC AAAAACCCTG AGGAAAGATG GAAAGAGCCT AGGCAAAGAC CTTCAGTTGT ACCAAGAGTC AAGATCTGCT TTTCTCGCGG TATCTCAGTT GTGTCAGGAT CAACCGGACA AGTGAGGACC CCTTCCATCA CTCACTGGCA CTCAGCACAA CACCCCCGCC CCGCCCACAG TGAGGTACAC CTTGCTAGAT GGGGGTCACC ATTCTCATTG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_010851
Summary	Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed:9697844). Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:9575168, PubMed:9697844). Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway (PubMed:9697844). Isoform 2 is defective in its ability to induce IRAK phosphorylation and NF-kappa-B activation and can function as a negative regulator of activation by IL-1 or lipopolysaccharide (LPS) (PubMed:11909531). Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes (PubMed:17018642). MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (PubMed:17635956, PubMed:21998396). Mediates leukocyte recruitment at the inflammatory site (PubMed:18941239).[UniProtKB/Swiss-Prot Function]

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