RHEB (NM_005614) Human Mass Spec Standard

Specifications

Product Data
Description	RHEB MS Standard C13 and N15-labeled recombinant protein (NP_005605)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC200307
Predicted MW	20.3 kDa
Protein Sequence	Sequence Link (showhide) >RC200307 representing NM_005614 Red=Cloning site Green=Tags(s) MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIF PQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAES WNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_005605
RefSeq Size	1396
RefSeq ORF	552
Synonyms	RHEB2
Locus ID	6009
Cytogenetics	7q36.1
Summary	This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008]
Protein Pathways	Insulin signaling pathway, mTOR signaling pathway

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