TFIIF (GTF2F1) (NM_002096) Human Mass Spec Standard

Specifications

Product Data
Description	GTF2F1 MS Standard C13 and N15-labeled recombinant protein (NP_002087)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC201294
Predicted MW	58.2 kDa
Protein Sequence	Sequence Link (showhide) >RC201294 protein sequence Red=Cloning site Green=Tags(s) MAALGPSSQNVTEYVVRVPKNTTKKYNIMAFNAADKVNFATWNQARLERDLSNKKIYQEEEMPESGAGSE FNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTENTSYYIFTQCPDGAFEAFPV HNWYNFTPLARHRTLTAEEAEEEWERRNKVLNHFSIMQQRRLKDQDQDEDEEEKEKRGRRKASELRIHDL EDDLEMSSDASDASGEEGGRVPKAKKKAPLAKGGRKKKKKKGSDDEAFEDSDDGDFEGQEVDYMSDGSSS SQEEPESKAKAPQQEEGPKGVDEQSDSSEESEEEKPPEEDKEEEEEKKAPTPQEKKRRKDSSEESDSSEE SDIDSEASSALFMAKKKTPPKRERKPSGGSSRGNSRPGTPSAEGGSTSSTLRAAASKLEQGKRVSEMPAA KRLRLDTGPQSLSGKSTPQPPSGKTTPNSGDVQVTEDAVRRYLTRKPMTTKDLLKKFQTKKTGLSSEQTV NVLAQILKRLNPERKMINDKMHFSLKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_002087
RefSeq Size	2551
RefSeq ORF	1551
Synonyms	BTF4; RAP74; TF2F1; TFIIF
Locus ID	2962
Cytogenetics	19p13.3
Summary	TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.[UniProtKB/Swiss-Prot Function]
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Basal transcription factors

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