UBL5 (NM_024292) Human Mass Spec Standard

Specifications

Product Data
Description	UBL5 MS Standard C13 and N15-labeled recombinant protein (NP_077268)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC202790
Predicted MW	8.5 kDa
Protein Sequence	Sequence Link (showhide) >RC202790 protein sequence Red=Cloning site Green=Tags(s) MIEVVCNDRLGKKVRVKCNTDDTIGDLKKLIAAQTGTRWNKIVLKKWYTIFKDHVSLGDYEIHDGMNLEL YYQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_077268
RefSeq Size	537
RefSeq ORF	219
Synonyms	HUB1
Locus ID	59286
Cytogenetics	19p13.2
Summary	This gene encodes a member of a group of proteins similar to ubiquitin. The encoded protein is not thought to degrade proteins like ubiquitin but to affect their function through being bound to target proteins by an isopeptide bond. The gene product has been studied as a link to predisposition to obesity based on its expression in Psammomys obesus, the fat sand rat, which is an animal model for obesity studies. Variation in this gene was found to be significantly associated with some metabolic traits (PMID: 15331561) but not associated with childhood obesity (PMID: 19189687). Pseudogenes of this gene are located on chromosomes 3, 5 and 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2013]

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