HLADQA1 (HLA-DQA1) (NM_002122) Human Mass Spec Standard

Specifications

Product Data
Description	HLA MS Standard C13 and N15-labeled recombinant protein (NP_002113)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC209921
Predicted MW	27.8 kDa
Protein Sequence	Sequence Link (showhide) >RC209921 protein sequence Red=Cloning site Green=Tags(s) MILNKALLLGALALTTVMSPCGGEDIVADHVASCGVNLYQFYGPSGQFTHEFDGDEQFYVDLEKKETAWR WPEFSKFGGFDPQGALRNMAVAKHNLNIMIKRYNSTAATNEVPEVTVFSKSPVTLGQPNTLICLDNIFPP VVNITWLSNGHAVTEGVSETSFLSKSDHSFFKISYLTFLPSADEIYDCKVEHWGLDQPLLKHWEPEIPAP MSELTETVVCALGLSVGLVGIVVGTVFIIQGLRSVGASRHQGPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_002113
RefSeq Size	1542
RefSeq ORF	762
Synonyms	CELIAC1; DQ-A1; DQA1; HLA-DQA
Locus ID	3117
Cytogenetics	6p21.32
Summary	HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq, Jul 2008]
Protein Families	Transmembrane
Protein Pathways	Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis

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