GCSH (NM_004483) Human Mass Spec Standard

Specifications

Product Data
Description	GCSH MS Standard C13 and N15-labeled recombinant protein (NP_004474)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC217886
Predicted MW	18.91 kDa
Protein Sequence	Sequence Link (showhide) >RC217886 representing NM_004483 Red=Cloning site Green=Tags(s) MALRVVRSVRALLCTLRAVPLPAAPCPPRPWQLGVGAVRTLRTGPALLSVRKFTEKHEWVTTENGIGTVG ISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAENPGLVNKSCYE DGWLIKMTLSNPSELDELMSEEAYEKYIKSIEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_004474
RefSeq Size	1161
RefSeq ORF	519
Synonyms	GCE; NKH
Locus ID	2653
Cytogenetics	16q23.2
Summary	Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH). Two transcript variants, one protein-coding and the other probably not protein-coding,have been found for this gene. Also, several transcribed and non-transcribed pseudogenes of this gene exist throughout the genome.[provided by RefSeq, Jan 2010]

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