NSMCE1 (NM_145080) Human Mass Spec Standard

CAT#: PH318073

NSMCE1 MS Standard C13 and N15-labeled recombinant protein (NP_659547)



  View other "NSMCE1" proteins (3)

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CNY 19,520.00


货期*
4周

规格
    • 10 ug

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经常一起买 (2)
Transient overexpression lysate of non-SMC element 1 homolog (S. cerevisiae) (NSMCE1)
    • 100 ug

CNY 3,080.00


Rabbit polyclonal antibody to NSMCE1 (non-SMC element 1 homolog (S. cerevisiae))
    • 100 ul

CNY 6,281.00

Specifications

Product Data
Description NSMCE1 MS Standard C13 and N15-labeled recombinant protein (NP_659547)
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence RC218073
Predicted MW 30.7 kDa
Protein Sequence
Tag C-Myc/DDK
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration >0.05 µg/µL as determined by microplate BCA method
Labeling Method Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq NP_659547
RefSeq Size 1079
RefSeq ORF 798
Synonyms NSE1
Locus ID 197370
Cytogenetics 16p12.1
Summary RING-type zinc finger-containing E3 ubiquitin ligase that assembles with melanoma antigen protein (MAGE) to catalyze the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. Within MAGE-RING ubiquitin ligase complex, MAGE stimulates and specifies ubiquitin ligase activity likely through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. Involved in maintenance of genome integrity, DNA damage response and DNA repair (PubMed:29225034, PubMed:20864041). NSMCE3/MAGEG1 and NSMCE1 ubiquitin ligase are components of SMC5-SMC6 complex and may positively regulate homologous recombination-mediated DNA repair (PubMed:18086888). MAGEF1-NSMCE1 ubiquitin ligase promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors (PubMed:29225034).[UniProtKB/Swiss-Prot Function]
Protein Families Druggable Genome
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