LCK (NM_005356) Human Mass Spec Standard

Specifications

Product Data
Description	LCK MS Standard C13 and N15-labeled recombinant protein (NP_005347)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC219375
Predicted MW	57.8 kDa
Protein Sequence	Sequence Link (showhide) RC219375
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_005347
RefSeq Size	2032
RefSeq ORF	1527
Synonyms	IMD22; LSK; p56lck; pp58lck; YT16
Locus ID	3932
Cytogenetics	1p35.2
Summary	This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Aug 2016]
Protein Families	Druggable Genome, Protein Kinase, Stem cell - Pluripotency
Protein Pathways	Natural killer cell mediated cytotoxicity, Primary immunodeficiency, T cell receptor signaling pathway

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