SAP155 (SF3B1) (NM_001005526) Human Mass Spec Standard

Specifications

Product Data
Description	SF3B1 MS Standard C13 and N15-labeled recombinant protein (NP_001005526)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC219649
Predicted MW	15.8 kDa
Protein Sequence	Sequence Link (showhide) >RC219649 representing NM_001005526 Red=Cloning site Green=Tags(s) MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDY SSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTMIISPERLDPFADGF YSAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_001005526
RefSeq Size	647
RefSeq ORF	432
Synonyms	Hsh155; MDS; PRP10; PRPF10; SAP155; SF3b155
Locus ID	23451
Cytogenetics	2q33.1
Summary	This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Protein Pathways	Spliceosome

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