JPT1 (NM_016185) Human Mass Spec Standard

Specifications

Product Data
Description	HN1 MS Standard C13 and N15-labeled recombinant protein (NP_057269)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC221865
Predicted MW	15.8 kDa
Protein Sequence	Sequence Link (showhide) >RC221865 representing NM_016185 Red=Cloning site Green=Tags(s) MTTTTTFKGVDPNSRNSSRVLRPPGGGSNFSLGFDEPTEQPVRKNKMASNIFGTPEENQASWAKSAGAKS SGGREDLESSGLQRRNSSEASSGDFLDLKGEGDIHENVDTDLPGSLGQSEEKPVPAAPVPSPVAPAPVPS RRNPPGGKSSLVLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_057269
RefSeq Size	1602
RefSeq ORF	462
Synonyms	ARM2; HN1; HN1A
Locus ID	51155
Cytogenetics	17q25.1
Summary	Modulates negatively AKT-mediated GSK3B signaling (PubMed:21323578, PubMed:22155408). Induces CTNNB1 'Ser-33' phosphorylation and degradation through the suppression of the inhibitory 'Ser-9' phosphorylation of GSK3B, which represses the function of the APC:CTNNB1:GSK3B complex and the interaction with CDH1/E-cadherin in adherent junctions (PubMed:25169422). Plays a role in the regulation of cell cycle and cell adhesion (PubMed:25169422, PubMed:25450365). Has an inhibitory role on AR-signaling pathway through the induction of receptor proteosomal degradation (PubMed:22155408).[UniProtKB/Swiss-Prot Function]

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