Cytokeratin 18 Bovine Protein

Specifications

Product Data
Description	Cytokeratin 18 bovine protein, 0.25 mg
Species	Bovine
Protein Source	Liver
Predicted MW	45 kDa
Concentration	lot specific
Purity	>95% (determined by SDS gelelectrophoresis)
Buffer	Presentation State: Purified State: Lyophilized Buffer System: 30 mM Tris/HCI pH 8, 9M urea, 2 mM DTT, 2 mM EDTA, 10 mM Methylammonium Chloride
Reconstitution	Restore with distilled water. BA1008S: 70 µl (final volume 100 µl). BA1008 : 175 µl (final volume 250 µl).
Preparation	Lyophilized
Applications	Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For Immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbeccos PBS). See References 2 and 3 for more details.
Protein Description	Bovine keratin K18 (formerly also designated Cytokeratin 18).
Note	Isoelectric Point: pI 5.4
Storage	Store at 2-8°C (lyophilized) and at -20°C (reconstituted). Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data
RefSeq	NP_000215
Locus ID	3875
Cytogenetics	12q13.13
Synonyms	Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18
Summary	KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Protein Families	Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For Immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbeccos PBS). See References 2 and 3 for more details.
Protein Pathways	Pathogenic Escherichia coli infection

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