Streptavidin Protein
CNY 4,270.00
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Specifications
Product Data | |
Description | Streptavidin protein, 1 mg |
Protein Source | S. avidinii |
Concentration | lot specific |
Purity | : Prepared from Chromatographically pure Streptavidin. |
Buffer | State: Lyophilized purifed Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium Azide as preservative. Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 Da). Absorption/Emission Wavelength: 495 nm/528 nm Fluorochrome/Protein Ratio: 2.2 moles FITC per mole of Streptavidin. Presentation Label: FITC |
Reconstitution | Restore with 1.0 ml of deionized water (or equivalent). |
Preparation | Lyophilized purifed |
Applications | Suitable for Immunofluorescence Microscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays. Recommended Dilutions: FLISA: 1/10,000-1/50,000. IF Microscopy: 1/1,000-1/5,000. Flow Cytometry: 1/500-1/2,500. |
Protein Description | Fluorescein Conjugated Streptavidin |
Note | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Streptavidin. |
Storage | Store vial at 2-8°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Reference Data | |
Summary | Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. |
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