Dnmt3b Mouse shRNA Plasmid (Locus ID 13436)

Specifications

Product Data
Product Name	Dnmt3b Mouse shRNA Plasmid (Locus ID 13436)
Locus ID	13436
UniProt ID	O88509
Synonyms	MmuIIIB
Vector	pRFP-C-RS
Format	Retroviral plasmids
Kit Components	Dnmt3b - Mouse, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 13436). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq	BC105677, BC105922, NM_001003960, NM_001003961, NM_001003963, NM_001122997, NM_001271744, NM_001271745, NM_001271746, NM_001271747, NM_010068, NM_001003961.1, NM_001003961.2, NM_001003961.3, NM_001003961.4, NM_001003960.1, NM_001003960.2, NM_001003960.3, NM_001003960.4, NM_010068.1, NM_010068.2, NM_010068.3, NM_010068.4, NM_010068.5, NM_001003963.1, NM_001003963.2, NM_001003963.3, NM_001003963.4, NM_001122997.1, NM_001122997.2, NM_001271747.1, NM_001271746.1, NM_001271745.1, NM_001271744.1
Summary	This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene results in severe developmental defects and loss of viability. Mutation of the related gene in humans causes immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. There is a pseudogene for this gene located adjacent to this gene in the same region of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.