WWP2 Human shRNA Plasmid Kit (Locus ID 11060)

Specifications

Product Data
Product Name	WWP2 Human shRNA Plasmid Kit (Locus ID 11060)
Locus ID	11060
UniProt ID	O00308
Synonyms	AIP2; WWp2-like
Vector	pGFP-V-RS
Format	Retroviral plasmids
Kit Components	WWP2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 11060). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq	NM_001270453, NM_001270454, NM_001270455, NM_007014, NM_199423, NM_199424, NM_199424.1, NM_199424.2, NM_007014.2, NM_007014.3, NM_007014.4, NM_001270455.1, NM_001270453.1, NM_001270454.1, NM_199423.1, BC064531, BC064531.1, BC000108, BC013645, NM_001270455.2, NM_001270454.2, NM_007014.5
Summary	This gene encodes a member of the Nedd4 family of E3 ligases, which play an important role in protein ubiquitination. The encoded protein contains four WW domains and may play a role in multiple processes including chondrogenesis and the regulation of oncogenic signaling pathways via interactions with Smad proteins and the tumor suppressor PTEN. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 10. [provided by RefSeq, Jul 2012]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.