SPHK2 Human shRNA Plasmid Kit (Locus ID 56848)

Specifications

Product Data
Product Name	SPHK2 Human shRNA Plasmid Kit (Locus ID 56848)
Locus ID	56848
UniProt ID	Q9NRA0
Synonyms	SK-2; SK 2; SPK-2; SPK 2
Vector	pGFP-V-RS
Format	Retroviral plasmids
Kit Components	SPHK2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 56848). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq	NM_001204158, NM_001204159, NM_001204160, NM_001243876, NM_020126, NM_020126.1, NM_020126.2, NM_020126.3, NM_020126.4, NM_001243876.1, NM_001204158.1, NM_001204158.2, NM_001204160.1, NM_001204160.2, NM_001204159.1, NM_001204159.2, BC006161, BC006161.1, BC010671, NM_001204158.3, NM_020126.5, NM_001204160.3, NM_001204159.3
Summary	This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.