RASSF1 Human shRNA Plasmid Kit (Locus ID 11186)

Specifications

Product Data
Product Name	RASSF1 Human shRNA Plasmid Kit (Locus ID 11186)
Locus ID	11186
UniProt ID	Q9NS23
Synonyms	123F2; NORE2A; RASSF1A; RDA32; REH3P21
Vector	pGFP-V-RS
Format	Retroviral plasmids
Kit Components	RASSF1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 11186). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq	NM_001206957, NM_007182, NM_170712, NM_170713, NM_170714, NM_170715, NM_170716, NM_170717, NM_170712.1, NM_170712.2, NM_170713.1, NM_170713.2, NM_007182.1, NM_007182.2, NM_007182.3, NM_007182.4, NM_170714.1, NM_001206957.1, NM_170715.1, NM_170716.1, NM_170717.1, BC110412, BC110412.1, BC117153, BC143879, BM152368, BM562405, NM_170713.3, NM_170714.2, NM_170712.3, NM_007182.5
Summary	This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, May 2011]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.