Sphk2 Mouse shRNA Plasmid (Locus ID 56632)

CAT#: TG503229

Sphk2 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided



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CNY 4,790.00


货期*
现货

规格
    • 1 kit

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TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00

Specifications

Product Data
Product Name Sphk2 Mouse shRNA Plasmid (Locus ID 56632)
Locus ID 56632
UniProt ID Q9JIA7
Synonyms C76851; Sk2; Spk2
Vector pGFP-V-RS
Format Retroviral plasmids
Kit Components Sphk2 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 56632). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq BC006941, BC053737, BC092084, NM_001172561, NM_020011, NM_203280, NM_020011.1, NM_020011.2, NM_020011.3, NM_020011.4, NM_020011.5, NM_001172561.1, NM_203280.1, NM_203280.2, NM_203280.3
Summary This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. The gene product also enhances apoptosis in different cell types and suppresses cellular proliferation. In mast cells, the encoded protein is necessary for influx of calcium, protein kinase C activation, and cytokine production and degranulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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