Mgea5 Mouse shRNA Plasmid (Locus ID 76055)
CAT#: TG505114
Mgea5 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
Need custom shRNA service?
Get a free quote
CNY 4,790.00
货期*
现货
规格
Cited in 2 publications. |
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
经常一起买 (1)
Specifications
Product Data | |
Product Name | Mgea5 Mouse shRNA Plasmid (Locus ID 76055) |
Locus ID | 76055 |
UniProt ID | Q9EQQ9 |
Synonyms | 2810009A20Rik; 4833427O07Rik; 5830447M11Rik; AA408215; Hy5; mKIAA0679; Ncoat; OGA |
Vector | pGFP-V-RS |
Format | Retroviral plasmids |
Kit Components | Mgea5 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 76055). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free. |
RefSeq | BC054821, NM_023799, NR_151594, NM_023799.1, NM_023799.2, NM_023799.3, BC020093, BC041109, BC147259, BC147260 |
Summary | Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:16517082). Does not bind acetyl-CoA and does not have histone acetyltransferase activity.[UniProtKB/Swiss-Prot Function] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
Citations (2)
The use of this RNAi has been cited in the following citations: |
---|
O-GlcNAcase targets pyruvate kinase M2 to regulate tumor growth
,null,
Oncogene
,PubMed ID 31501520
[Oga]
|
Thiamet-G-mediated inhibition of O-GlcNAcase sensitizes human leukemia cells to microtubule-stabilizing agent paclitaxel.
,null,
Biochemical and biophysical research communications
,PubMed ID 25268318
[Oga]
|
Documents
Product Manuals |
FAQs |
SDS |
Customer
Reviews
Loading...