March5 Mouse shRNA Plasmid (Locus ID 69104)

Specifications

Product Data
Product Name	March5 Mouse shRNA Plasmid (Locus ID 69104)
Locus ID	69104
UniProt ID	Q3KNM2
Synonyms	1810015H18Rik; 2310008I22Rik; 2700055A20Rik; 5730499H23Rik; E130202O05Rik; MARCH-V
Vector	pGFP-V-RS
Format	Retroviral plasmids
Kit Components	March5 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 69104). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq	BC064752, BC107215, BC132455, BC132457, NM_001164336, NM_001164337, NM_027314, NM_027314.1, NM_027314.2, NM_027314.3, NM_001164336.1, NM_001164337.1, BC107214
Summary	Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.