IKZF3 Human shRNA Plasmid Kit (Locus ID 22806)

Specifications

Product Data
Product Name	IKZF3 Human shRNA Plasmid Kit (Locus ID 22806)
Locus ID	22806
UniProt ID	Q9UKT9
Synonyms	AIO; AIOLOS; ZNFN1A3
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	IKZF3 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 22806). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001257408, NM_001257409, NM_001257410, NM_001257411, NM_001257412, NM_001257413, NM_001257414, NM_001284514, NM_001284515, NM_001284516, NM_012481, NM_183228, NM_183229, NM_183230, NM_183231, NM_183232, NR_047559, NR_047560, NR_047561, NM_012481.1, NM_012481.2, NM_012481.3, NM_012481.4, NM_183230.1, NM_183230.2, NM_183228.1, NM_183228.2, NM_183229.1, NM_183229.2, NM_183231.1, NM_183232.1, NM_183232.2, NM_001257413.1, NM_001257412.1, NM_001257411.1, NM_001257410.1, NM_001257414.1, NM_001257409.1, NM_001257408.1, NM_001284514.1, NM_001284515.1, NM_001284516.1, BC032707, BC032707.1, NM_183230.3, NM_183232.3, NM_001257412.2, NM_001257410.2, NM_012481.5, NM_001284515.2, NM_001257408.2, NM_001257409.2, NM_183229.3, NM_001257413.2, NM_183228.3, NM_183231.3, NM_001257411.2, NM_001257414.2
Summary	This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. Several alternative transcripts encoding different isoforms have been described, as well as some non-protein coding variants. [provided by RefSeq, Apr 2012]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.