LEF1 Human shRNA Plasmid Kit (Locus ID 51176)

Specifications

Product Data
Product Name	LEF1 Human shRNA Plasmid Kit (Locus ID 51176)
Locus ID	51176
UniProt ID	Q9UJU2
Synonyms	LEF-1; TCF1ALPHA; TCF7L3; TCF10
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	LEF1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 51176). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001130713, NM_001130714, NM_001166119, NM_016269, NM_016269.1, NM_016269.2, NM_016269.3, NM_016269.4, NM_001130713.1, NM_001130713.2, NM_001130714.1, NM_001130714.2, NM_001166119.1, BC050632, BC050632.2, BC040559, NM_016269.5
Summary	This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.