DOK7 Human shRNA Plasmid Kit (Locus ID 285489)

Specifications

Product Data
Product Name	DOK7 Human shRNA Plasmid Kit (Locus ID 285489)
Locus ID	285489
UniProt ID	Q18PE1
Synonyms	C4orf25; CMS1B; CMS10; FADS3
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	DOK7 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 285489). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001164673, NM_001256896, NM_001301071, NM_173660, NM_173660.1, NM_173660.2, NM_173660.3, NM_173660.4, NM_001164673.1, NM_001256896.1, NM_001301071.1, BC131544, BC043568, BC062369, BC141852, BM684814, NM_001363811, NM_001301071.2, NM_001164673.2, NM_173660.5
Summary	The protein encoded by this gene is essential for neuromuscular synaptogenesis. The protein functions in aneural activation of muscle-specific receptor kinase, which is required for postsynaptic differentiation, and in the subsequent clustering of the acetylcholine receptor in myotubes. This protein can also induce autophosphorylation of muscle-specific receptor kinase. Mutations in this gene are a cause of familial limb-girdle myasthenia autosomal recessive, which is also known as congenital myasthenic syndrome type 1B. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.