Beta Arrestin 2 (ARRB2) Human shRNA Plasmid Kit (Locus ID 409)

Specifications

Product Data
Product Name	Beta Arrestin 2 (ARRB2) Human shRNA Plasmid Kit (Locus ID 409)
Locus ID	409
UniProt ID	P32121
Synonyms	ARB2; ARR2; BARR2
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	ARRB2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 409). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001257328, NM_001257329, NM_001257330, NM_001257331, NM_004313, NM_199004, NR_047516, NM_001330064, NM_004313.1, NM_004313.2, NM_004313.3, NM_199004.1, NM_001257329.1, NM_001257331.1, NM_001257330.1, NM_001257328.1, BC007427, BC007427.2, BC039066, BC067368, BC095450, NM_199004.2, NM_001257328.2, NM_001257329.2, NM_001257331.2, NM_001257330.2
Summary	Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.