SEPT4 Human shRNA Plasmid Kit (Locus ID 5414)

Specifications

Product Data
Product Name	SEPT4 Human shRNA Plasmid Kit (Locus ID 5414)
Locus ID	5414
UniProt ID	O43236
Synonyms	ARTS; BRADEION; C17orf47; CE5B3; H5; hCDCREL-2; hucep-7; MART; PNUTL2; SEP4; SEPT4
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	SEPT4 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 5414). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001038704, NM_001198713, NM_001256782, NM_001256822, NM_004574, NM_080415, NM_080416, NM_080417, NR_037155, NR_037156, NR_104196, NR_104197, NM_080416.1, NM_080416.2, NM_080416.3, NM_001038704.2, NM_004574.1, NM_004574.2, NM_004574.3, NM_004574.4, NM_080415.1, NM_080415.2, NM_080415.3, NM_001198713.1, NM_001256822.1, NM_001256782.1, BC018056, BC018056.1, BC018055, BC128255, BC128515, BC142968, NM_001363803, NM_001368771, NM_001368772
Summary	This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer. [provided by RefSeq, Nov 2010]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.