TEA domain family member 2 (TEAD2) Human shRNA Plasmid Kit (Locus ID 8463)

Specifications

Product Data
Product Name	TEA domain family member 2 (TEAD2) Human shRNA Plasmid Kit (Locus ID 8463)
Locus ID	8463
UniProt ID	Q15562
Synonyms	ETF; TEAD-2; TEF-4; TEF4
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	TEAD2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 8463). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001256658, NM_001256659, NM_001256660, NM_001256661, NM_001256662, NM_003598, NM_003598.1, NM_001256662.1, NM_001256658.1, NM_001256659.1, NM_001256660.1, NM_001256661.1, BC007556, BC007556.1, BC018803, BC051301, NM_001256662.2, NM_003598.2, NM_001256661.2, NM_001256658.2, NM_001256660.2, NM_001256659.2
Summary	Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.