PFKP Human shRNA Plasmid Kit (Locus ID 5214)

Specifications

Product Data
Product Name	PFKP Human shRNA Plasmid Kit (Locus ID 5214)
Locus ID	5214
UniProt ID	Q01813
Synonyms	ATP-PFK; PFK-C; PFK-P; PFKF
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	PFKP - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 5214). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001242339, NM_002627, NM_001323067, NM_001323068, NM_001323069, NM_001323070, NM_001323071, NM_001323072, NM_001323073, NM_001323074, NM_001345944, NM_002627.1, NM_002627.2, NM_002627.3, NM_002627.4, NM_001242339.1, BC002536, BC002536.1, BC021698, BC029138, BC043156
Summary	This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.