Nucleoside phosphorylase (PNP) Human shRNA Plasmid Kit (Locus ID 4860)

CAT#: TL311133

PNP - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided



Need custom shRNA service?
Get a free quote

CNY 5,740.00


货期*
现货

规格
    • 1 kit

Product images

经常一起买 (4)
Lenti-vpak packaging kit - packaging plasmids and transfection reagent
    • 10 reactions

CNY 5,420.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Rabbit Polyclonal Anti-NP Antibody
    • 50 ug

CNY 4,628.00


Lateral flow testing sticks used for the semi-quantitative detection of the lentiviral p24 protein, 20 tests
    • 20 Tests

CNY 4,070.00

Specifications

Product Data
Product Name Nucleoside phosphorylase (PNP) Human shRNA Plasmid Kit (Locus ID 4860)
Locus ID 4860
UniProt ID P00491
Synonyms NP; PRO1837; PUNP
Vector pGFP-C-shLenti
Format Lentiviral plasmids
Kit Components PNP - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 4860). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq NM_000270, NM_000270.1, NM_000270.2, NM_000270.3, BC106074, BC106074.1, BC104206, BC104207, BM193354, NM_000270.4
Summary This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Jul 2008]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...