LAMA3 Human shRNA Plasmid Kit (Locus ID 3909)

Specifications

Product Data
Product Name	LAMA3 Human shRNA Plasmid Kit (Locus ID 3909)
Locus ID	3909
UniProt ID	Q16787
Synonyms	BM600; E170; LAMNA; LOCS
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	LAMA3 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 3909). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_000227, NM_001127717, NM_001127718, NM_001302996, NM_198129, NR_130106, NM_000227.1, NM_000227.2, NM_000227.3, NM_000227.4, NM_198129.1, NM_198129.2, NM_001127718.1, NM_001127718.2, NM_001127717.1, NM_001127717.2, NM_001302996.1, BC033663, BC043618, BC093406, BC172402, BC172580, NM_001302996.2
Summary	The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.