Hsp40 (DNAJB1) Human shRNA Plasmid Kit (Locus ID 3337)
CAT#: TL313427
DNAJB1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
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CNY 5,740.00
Cited in 1 publication. |
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CNY 1,999.00
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CNY 4,070.00
Specifications
Product Data | |
Product Name | Hsp40 (DNAJB1) Human shRNA Plasmid Kit (Locus ID 3337) |
Locus ID | 3337 |
UniProt ID | P25685 |
Synonyms | Hdj1; Hsp40; HSPF1; RSPH16B; Sis1 |
Vector | pGFP-C-shLenti |
Format | Lentiviral plasmids |
Kit Components | DNAJB1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 3337). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free. |
RefSeq | NM_001300914, NM_001313964, NM_006145, NM_006145.1, NM_006145.2, NM_001300914.1, BC002352, BC002352.2, BC013157, BC019827, BM795601, NM_006145.3 |
Summary | This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
Citations (1)
The use of this RNAi has been cited in the following citations: |
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FOXE3 contributes to Peters anomaly through transcriptional regulation of an autophagy-associated protein termed DNAJB1
,Khan, SY;Vasanth, S;Kabir, F;Gottsch, JD;,
Nature Communications
,PubMed ID 27218149
[DNAJB1]
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