CNOT8 Human shRNA Plasmid Kit (Locus ID 9337)

Specifications

Product Data
Product Name	CNOT8 Human shRNA Plasmid Kit (Locus ID 9337)
Locus ID	9337
UniProt ID	Q9UFF9
Synonyms	CAF1; Caf1b; CALIF; hCAF1; POP2
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	CNOT8 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 9337). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001301073, NM_001301074, NM_001301075, NM_001301077, NM_001301080, NM_001301082, NM_001301083, NM_001301086, NM_004779, NM_004779.1, NM_004779.2, NM_004779.3, NM_004779.4, NM_004779.5, NM_001301086.1, NM_001301075.1, NM_001301077.1, NM_001301080.1, NM_001301082.1, NM_001301083.1, NM_001301074.1, NM_001301073.1, BC017366, BC017366.2, BC093025, NM_001301080.2, NM_001301086.2, NM_001301073.2, NM_001301077.2, NM_001301074.2, NM_004779.6, NM_001301075.2
Summary	Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT7. Catalytic component of the CCR4-NOT complex which is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.