PIGA Human shRNA Plasmid Kit (Locus ID 5277)

CAT#: TL316656

PIGA - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided



Need custom shRNA service?
Get a free quote

CNY 7,740.00


货期*
2周

规格
    • 1 kit

Product images

经常一起买 (4)
Lenti-vpak packaging kit - packaging plasmids and transfection reagent
    • 10 reactions

CNY 5,420.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


PIGA Antibody - middle region
    • 100 ul

CNY 5,250.00


Lateral flow testing sticks used for the semi-quantitative detection of the lentiviral p24 protein, 20 tests
    • 20 Tests

CNY 4,070.00

Specifications

Product Data
Product Name PIGA Human shRNA Plasmid Kit (Locus ID 5277)
Locus ID 5277
UniProt ID P37287
Synonyms GPI3; MCAHS2; PIG-A; PNH1
Vector pGFP-C-shLenti
Format Lentiviral plasmids
Kit Components PIGA - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 5277). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq NM_002641, NM_020472, NM_020473, NR_033835, NR_033836, NM_020473.1, NM_020473.2, NM_020473.3, NM_002641.1, NM_002641.2, NM_002641.3, NM_020472.1, BC038236, BM998465, NM_002641.4
Summary This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. A related pseudogene is located on chromosome 12. [provided by RefSeq, Jun 2010]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...