Neurofascin (NFASC) Human shRNA Plasmid Kit (Locus ID 23114)

CAT#: TL316992

NFASC - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided



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CNY 7,740.00


货期*
2周

规格
    • 1 kit

Product images

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Lateral flow testing sticks used for the semi-quantitative detection of the lentiviral p24 protein, 20 tests
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Specifications

Product Data
Product Name Neurofascin (NFASC) Human shRNA Plasmid Kit (Locus ID 23114)
Locus ID 23114
UniProt ID O94856
Synonyms NF, NRCAML, FLJ46866, KIAA0756, DKFZp686P2250
Vector pGFP-C-shLenti
Format Lentiviral plasmids
Kit Components NFASC - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 23114). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq NM_001005387, NM_001005388, NM_001005389, NM_001160331, NM_001160332, NM_001160333, NM_015090, NM_015090.1, NM_015090.2, NM_015090.3, NM_001160333.1, NM_001005389.1, NM_001160332.1, NM_001160331.1, NM_001005388.1, NM_001005388.2, BC144454, BC008124, BC033367, BC035389, BC040674, BC117674, BC137013, BC144455, NM_001365986, NM_001160332.2, NM_001160333.2, NM_001005389.2, NM_015090.4
Summary This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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