CHST15 Human shRNA Plasmid Kit (Locus ID 51363)

Specifications

Product Data
Product Name	CHST15 Human shRNA Plasmid Kit (Locus ID 51363)
Locus ID	51363
UniProt ID	Q7LFX5
Synonyms	BRAG; GALNAC4S-6ST
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	CHST15 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 51363). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001270764, NM_001270765, NM_014863, NM_015892, NM_015892.1, NM_015892.2, NM_015892.3, NM_015892.4, NM_014863.1, NM_014863.2, NM_014863.3, NM_001270764.1, NM_001270765.1, BC027908, BC027908.1, BC050540, BC075813, BM193389, BM977716, NM_001270764.2, NM_001270765.2, NM_014863.4, NM_015892.5
Summary	Chondroitin sulfate (CS) is a glycosaminoglycan which is an important structural component of the extracellular matrix and which links to proteins to form proteoglycans. Chondroitin sulfate E (CS-E) is an isomer of chondroitin sulfate in which the C-4 and C-6 hydroxyl groups are sulfated. This gene encodes a type II transmembrane glycoprotein that acts as a sulfotransferase to transfer sulfate to the C-6 hydroxal group of chondroitin sulfate. This gene has also been identified as being co-expressed with RAG1 in B-cells and as potentially acting as a B-cell surface signaling receptor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.