Phf21a Mouse shRNA Plasmid (Locus ID 192285)

Specifications

Product Data
Product Name	Phf21a Mouse shRNA Plasmid (Locus ID 192285)
Locus ID	192285
UniProt ID	Q6ZPK0
Synonyms	80kDa; Bhc; Bhc80; D030065N23Rik; PFTF1
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	Phf21a - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 192285). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	BC019181, BC106805, NM_001109690, NM_001109691, NM_001346704, NM_138755, NM_138755.1, NM_138755.2, NM_001109691.1, NM_001109690.1, NM_001362829, NM_001362830, NM_001362831, NM_001362832, NM_001362833, NM_001109690.2
Summary	Component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation (By similarity).[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.