Zfp322a Mouse shRNA Plasmid (Locus ID 218100)

Specifications

Product Data
Product Name	Zfp322a Mouse shRNA Plasmid (Locus ID 218100)
Locus ID	218100
UniProt ID	Q8BZ89
Synonyms	Znf322; Znf322a
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	Zfp322a - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 218100). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	BC043711, NM_001111107, NM_001286155, NM_001286156, NM_172586, NM_001111107.1, NM_001111107.2, NM_172586.1, NM_172586.2, NM_172586.3, NM_172586.4, NM_001286155.1, NM_001286156.1, BC017143, BC032268
Summary	Transcriptional activator. Important for maintenance of pluripotency in embryonic stem cells. Binds directly to the POU5F1 distal enhancer and the NANOG proximal promoter, and enhances expression of both genes. Can also bind to numerous other gene promoters and regulates expression of many other pluripotency factors, either directly or indirectly. Promotes inhibition of MAPK signaling during embryonic stem cell differentiation.[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.