Dclk2 Mouse shRNA Plasmid (Locus ID 70762)

Specifications

Product Data
Product Name	Dclk2 Mouse shRNA Plasmid (Locus ID 70762)
Locus ID	70762
UniProt ID	Q6PGN3
Synonyms	6330415M09Rik; AU044875; CL2; Clic; Click-II; CLICK2; Dcamk; Dcamkl2
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	Dclk2 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 70762). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	BC056921, NM_001195496, NM_001195497, NM_001195498, NM_001195499, NM_001195500, NM_027539, NM_001195497.1, NM_027539.1, NM_027539.2, NM_027539.3, NM_027539.4, NM_027539.5, NM_001195496.1, NM_001195498.1, NM_001195499.1, NM_001195500.1, BC052071, BC060192, NM_001195500.2
Summary	This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. This gene and the DCX gene, another family member, share function in the establishment of hippocampal organization and their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.