Vamp7 Mouse shRNA Plasmid (Locus ID 20955)

CAT#: TL514022

Vamp7 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided



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CNY 7,740.00


货期*
2周

规格
    • 1 kit

Product images

经常一起买 (4)
Lenti-vpak packaging kit - packaging plasmids and transfection reagent
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TurboFectin Transfection Reagent (1 mL in 1 vial)
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Vamp7 Antibody - C-terminal region
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Lateral flow testing sticks used for the semi-quantitative detection of the lentiviral p24 protein, 20 tests
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Specifications

Product Data
Product Name Vamp7 Mouse shRNA Plasmid (Locus ID 20955)
Locus ID 20955
UniProt ID P70280
Synonyms Sybl1; TI-VAMP; VAMP-7
Vector pGFP-C-shLenti
Format Lentiviral plasmids
Kit Components Vamp7 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 20955). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq BC003764, NM_001302138, NM_011515, NM_001359151, NM_011515.1, NM_011515.2, NM_011515.3, NM_011515.4, NM_011515.5, NM_001302138.1
Summary Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.[UniProtKB/Swiss-Prot Function]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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