Traf3ip2 Mouse shRNA Plasmid (Locus ID 103213)

Specifications

Product Data
Product Name	Traf3ip2 Mouse shRNA Plasmid (Locus ID 103213)
Locus ID	103213
UniProt ID	Q8N7N6
Synonyms	Act1; AI429613; CIKS
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	Traf3ip2 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 103213). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	BC096483, NM_134000, NM_134000.1, NM_134000.2, NM_134000.3, BC008534, BC008535, BC022633, BC071207, BC138234, BC138235
Summary	E3 ubiquitin ligase that catalyzes 'Lys63'-linked polyubiquitination of target protein, enhancing protein-protein interaction and cell signaling (By similarity). Transfers ubiquitin from E2 ubiquitin-conjugating enzyme UBE2V1-UBE2N to substrate protein (By similarity). Essential adapter molecule in IL17A-mediated signaling (PubMed:19825828). Upon IL17A stimulation, interacts with IL17RA and IL17RC receptor chains through SEFIR domains and catalyzes 'Lys63'-linked polyubiquitination of TRAF6, leading to TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways (PubMed:19825828).[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.