Dnajb11 Mouse shRNA Plasmid (Locus ID 67838)

Specifications

Product Data
Product Name	Dnajb11 Mouse shRNA Plasmid (Locus ID 67838)
Locus ID	67838
UniProt ID	Q99KV1
Synonyms	1810031F23Rik; ABBP-2; AL024055; Dj9; ERdj3; ERj3p
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	Dnajb11 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 67838). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	BC003999, BC018282, BC040747, NM_001190804, NM_001190805, NM_026400, NM_001190805.1, NM_026400.1, NM_026400.2, NM_026400.3, NM_026400.4, NM_026400.5, NM_001190804.1
Summary	As a co-chaperone for HSPA5 it is required for proper folding, trafficking or degradation of proteins. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity. It is necessary for maturation and correct trafficking of PKD1.[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.