TXNRD1 Human shRNA Plasmid Kit (Locus ID 7296)

Specifications

Product Data
Product Name	TXNRD1 Human shRNA Plasmid Kit (Locus ID 7296)
Locus ID	7296
UniProt ID	Q16881
Synonyms	GRIM-12; TR; TR1; TRXR1; TXNR
Vector	pRS
Format	Retroviral plasmids
Kit Components	TXNRD1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 7296). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001093771, NM_001261445, NM_001261446, NM_003330, NM_182729, NM_182742, NM_182743, NM_003330.1, NM_003330.2, NM_003330.3, NM_001093771.1, NM_001093771.2, NM_182729.1, NM_182729.2, NM_182743.1, NM_182743.2, NM_182742.1, NM_182742.2, NM_001261446.1, NM_001261445.1, BC018122, BC018122.1, BC042974, NM_001093771.3, NM_001261445.2, NM_182743.3, NM_182729.3, NM_001261446.2, NM_182742.3, NM_003330.4
Summary	The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms, including a glutaredoxin-containing isoform that is predominantly expressed in testis. [provided by RefSeq, May 2017]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.