RAD17 Human shRNA Plasmid Kit (Locus ID 5884)

Specifications

Product Data
Product Name	RAD17 Human shRNA Plasmid Kit (Locus ID 5884)
Locus ID	5884
UniProt ID	O75943
Synonyms	CCYC; HRAD17; R24L; RAD17SP; RAD24
Vector	pRS
Format	Retroviral plasmids
Kit Components	RAD17 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 5884). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001278622, NM_002873, NM_133338, NM_133339, NM_133340, NM_133341, NM_133342, NM_133343, NM_133344, NM_002873.1, NM_133340.1, NM_133340.2, NM_133343.1, NM_133344.1, NM_133344.2, NM_133342.1, NM_133342.2, NM_133339.1, NM_133339.2, NM_133341.1, NM_133341.2, NM_133338.1, NM_133338.2, NM_001278622.1, BC032304, BC032304.1, BC018110, NM_133338.3, NM_133344.3, NM_133342.3
Summary	The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.