OGG1 Human shRNA Plasmid Kit (Locus ID 4968)

Specifications

Product Data
Product Name	OGG1 Human shRNA Plasmid Kit (Locus ID 4968)
Locus ID	4968
UniProt ID	O15527
Synonyms	HMMH; HOGG1; MUTM; OGH1
Vector	pRS
Format	Retroviral plasmids
Kit Components	OGG1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 4968). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_002542, NM_016819, NM_016820, NM_016821, NM_016826, NM_016827, NM_016828, NM_016829, NM_001354648, NM_001354649, NM_001354650, NM_001354651, NM_001354652, NR_148930, NR_148931, NR_148932, NM_002542.1, NM_002542.2, NM_002542.3, NM_002542.4, NM_002542.5, NM_016819.1, NM_016819.2, NM_016819.3, NM_016827.1, NM_016827.2, NM_016820.1, NM_016820.2, NM_016820.3, NM_016821.1, NM_016821.2, NM_016826.1, NM_016826.2, NM_016828.1, NM_016828.2, NM_016829.1, NM_016829.2, BC000657, BC000657.2, BM847736
Summary	This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.