MEST Human shRNA Plasmid Kit (Locus ID 4232)

Specifications

Product Data
Product Name	MEST Human shRNA Plasmid Kit (Locus ID 4232)
Locus ID	4232
UniProt ID	Q5EB52
Synonyms	PEG1
Vector	pRS
Format	Retroviral plasmids
Kit Components	MEST - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 4232). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001253900, NM_001253901, NM_001253902, NM_002402, NM_177524, NM_177525, NM_002402.1, NM_002402.2, NM_002402.3, NM_177524.1, NM_177524.2, NM_177525.1, NM_177525.2, NM_001253901.1, NM_001253902.1, NM_001253900.1, BC014564, BC014564.2, BC002413, BC011908, BC018695, BC090049, NM_002402.4
Summary	This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15. [provided by RefSeq, Dec 2011]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.