CD130 (IL6ST) Human shRNA Plasmid Kit (Locus ID 3572)

Specifications

Product Data
Product Name	CD130 (IL6ST) Human shRNA Plasmid Kit (Locus ID 3572)
Locus ID	3572
UniProt ID	P40189
Synonyms	CD130; CDW130; GP130; HIES4; IL-6RB; sGP130
Vector	pRS
Format	Retroviral plasmids
Kit Components	IL6ST - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 3572). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001190981, NM_002184, NM_175767, NR_120480, NM_002184.1, NM_002184.2, NM_002184.3, NM_175767.1, NM_175767.2, NM_001190981.1, BC071555, BC117402, BC117404, BC143842, NM_001364276, NM_001364278, NR_157112, NM_001364275, NM_001364277, NM_001364279, NM_002184.4, NM_001190981.2, NM_175767.3
Summary	The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.