ERCC1 Human shRNA Plasmid Kit (Locus ID 2067)

Specifications

Product Data
Product Name	ERCC1 Human shRNA Plasmid Kit (Locus ID 2067)
Locus ID	2067
UniProt ID	P07992
Synonyms	COFS4; RAD10; UV20
Vector	pRS
Format	Retroviral plasmids
Kit Components	ERCC1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 2067). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001166049, NM_001983, NM_202001, NM_001983.1, NM_001983.2, NM_001983.3, NM_202001.1, NM_202001.2, NM_001166049.1, BC008930, BC008930.2, BC052813, BC052813.1, BM011724, BM450988, BM789972, NM_001369409, NM_001369414, NM_001369416, NM_001369419, NM_001369408, NM_001369410, NM_001369411, NM_001369412, NM_001369413, NM_001369415, NM_001369417, NM_001369418, NM_001166049.2, NM_202001.3
Summary	The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.